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Description:Measurements on standards showed a linear response between 0 and By taking advantage of surface functionalization techniques, one microsphere acts as a dynamic reference, compensating for nonspecific binding events and changes in the environment such as refractive index and temperature , while the other microsphere is functionalized to detect a specific interaction. Measurements performed on 16 whole blood samples from mixed breeds showed good correlation with a commercially available ELISA assay. This ensures their responses to nonspecific binding and environmental changes are almost identical, whereby any specific changes, such as binding events, can be monitored via the relative movement between the two sets of WGM peaks. The MOF allows remote excitation and collection of the whispering gallery modes WGMs of the microspheres while also providing a robust and easy to manipulate dip-sensing platform. The almost identical size allows the two spheres to have virtually identical refractive index sensitivity and surface area, while still having discernible WGM spectra.

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